Cryopreservation vs. vitrification. When Assisted Reproductive Technology (ART) began to be popular, freezing or cryopreservation, also known as slow freezing, was the only technique available. Since this method offered high success rates in the case of sperm cryopreservation, today it continues to be the technique used for preserving sperm cells.
The use of vitrification for purified sperm samples could potentially result in a superior post-thaw quality of spermatozoa for ART procedures, with positive economic and time implications.
Sperm vitrification Campus Granada Spain With permission from V. Isachenko • Direct contact between of cells / tissue with liquid nitrogen • Vitrificacion eliminates crystallization totally • Fast methods: vitrification / thaw • Using small volumes for freezing it is improve the rate of freezing.
This vitrification technique that preserves most of the sperm cell functions is an easy, speedy, and cheap alternative for sperm bank cryopreservation. Vitrification of Human Spermatozoa Without Cryoprotectants.
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